Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier

• Aniela Bittner,
• Angélique D. Ducray,
• Hans Rudolf Widmer,
• Michael H. Stoffel and
• Meike Mevissen

Beilstein J. Nanotechnol. 2019, 10, 941–954, doi:10.3762/bjnano.10.95

• by both cell types. However, uptake was found to be more prominent in RBE4 cells compared to MDCK II cells [18]. After exposure of rat primary cultured brain microvessel ECs (rBMECs) to Au-NPs, smaller NPs were demonstrated to be taken up to a higher extent compared to larger NPs. Overall, only the

• cells containing NPs. Similar to our findings, mesoporous Si-NPs were taken up by both epithelial, MDCK II, and endothelial cells, RBE4. Uptake was shown to be enhanced by copolymer coating and was found more prominent in RBE4 cells compared to MDCK II cells [18]. In contrast to our findings, Trickler

The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots

• Vladimir V. Breus,
• Anna Pietuch,
• Marco Tarantola,
• Thomas Basché and
• Andreas Janshoff

Beilstein J. Nanotechnol. 2015, 6, 281–292, doi:10.3762/bjnano.6.26

• II cells after 6 h of exposure. We used chlorpromazine to inhibit the formation of clathrin-coated pits through the assembly of clathrin and AP2 adaptor complexes in the endosomal membranes, 5-(N,N-dimethyl)amiloride hydrochloride (DMA). This prohibited macropinocytosis by blocking amiloride

• interior during the first 6 h after addition of QDs. For longer exposure times, the cell interior close to the nucleus displays more particles (Figure 3c). In order to elucidate the mechanism of QD internalization by MDCKII cells, we studied nonspecific interactions between QDs and inhibitor-treated MDCK

Mechanical properties of MDCK II cells exposed to gold nanorods

• Anna Pietuch,
• Bastian Rouven Brückner,
• David Schneider,
• Marco Tarantola,
• Christina Rosman,
• Carsten Sönnichsen and
• Andreas Janshoff

Beilstein J. Nanotechnol. 2015, 6, 223–231, doi:10.3762/bjnano.6.21

• the cellular response to external stimuli. Results: Mechanical properties of confluent MDCK II cells exposed to gold nanorods as a function of surface functionalization and concentration have been explored by atomic force microscopy and quartz crystal microbalance measurements in combination with

• CTAB coated rods suggesting an increase in acoustic load corresponding to a larger stiffness (storage modulus). Keywords: atomic force microscopy; CTAB; gold nanorods; membrane tension; MDCK II cells; QCM; Introduction The interest in gold nanoparticles (NP) for biomedical applications in the field

• the elasticity of the epithelial cell line MDCK II probed by AFM and QCM. The combination of these two techniques allows to monitor the influence of nanoparticles on the elastic properties of MDCK II cells both from the apical and basal side. The data permits to compare mechanics of cells exposed to

Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating

• Christina Rosman,
• Sebastien Pierrat,
• Marco Tarantola,
• David Schneider,
• Eva Sunnick,
• Andreas Janshoff and
• Carsten Sönnichsen

Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257

• special functionality), this study is focused on the impact of basolateral exposure of gold nanoparticles on epithelial cells. Here, epithelial cells were exposed to nanoparticles adsorbed onto a surface. Since MDCK II cells exhibit caveolae only basolaterally, it is conceivable that internalization is

• apical application of the same functionalized nanoparticles are presented elsewhere [18][19][20]. Results System We investigate the growth of mammalian epithelial kidney (MDCK II) cells seeded on glass substrates decorated with gold nanorods (38 × 17 nm, Supporting Information of [20]) and compare the

• obtained from gel electrophoresis for PEG particles can be found in the Supporting Information of [20]. Live cell imaging In order to investigate how single MDCK II cells adhere and grow on nanoparticle-decorated substrates, live cell imaging using optical dark field microscopy was performed. For